Structure of the human GABARAPL2 protein in complex with the UBA5 LIR motif

Gamma-aminobutyric acid receptor-associated protein-like 2, Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif

3D NOESY-[13C,1H]-HSQC 13C/15N filtered in F1

An atypical LIR motif within UBA5 (ubiquitin like modifier activating enzyme 5) interacts with GABARAP proteins and mediates membrane localization of UBA5.

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2) residues 3-116, residues 1-2 are expressing tag

UFM1-activating enzyme 5 (UBA5) LIR/UFIM containing peptide (resides 333-348). First 3 residues (Gly-Ala-Met) are expression tag.

GABA(A) receptor-associated protein-like 2

Golgi-associated ATPase enhancer of 16 kDa

Ubiquitin-activating enzyme E1 domain-containing protein 1

0.6 mM [U-99% 13C; U-99% 15N] GABARAPL2, 1.0 mM No Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif,
50 mM No sodium phosphate, 100 mM No sodium chloride, 4.6 mM No sodium azide, 95% H2O/5% D2O

1.0 mM No GABARAPL2, 0.6 mM [U-99% 13C; U-99% 15N] Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif,
50 mM No sodium phosphate, 100 mM No sodium chloride, 4.6 mM No sodium azide, 95% H2O/5% D2O

Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif
Luginbuhl, Guntert, Billeter and Wuthrich