Solution NMR structure of human DNMT1 N-terminal alpha-helical domain

DNA (cytosine-5)-methyltransferase 1 (E.C.2.1.1.37)

Identification of a conserved α-helical domain at the N-terminus of human DNA methyl transferase 1.

1 mM [U-13C; U-15N] DNMT1, 20 mM MES/Bis-Tris, 50 mM sodium chloride, 90% H2O/10% D2O.

1 mM [U-15N] DNMT1, 20 mM MES/Bis-Tris, 50 mM sodium chloride, 90% H2O/10% D2O.

1 mM [U-13C; U-15N] DNMT1, 20 mM MES/Bis-Tris, 50 mM sodium chloride, 100% D2O.

1 mM [U-15N] DNMT1, 20 mM MES/Bis-Tris, 50 mM sodium chloride, 5 % pentaethylene glycol monododecyl ether (C12E5), 95 % n-hexanol, 90% H2O/10% D2O.

pentaethylene glycol monododecyl ether (C12E5)

Schwieters, Kuszewski, Tjandra and Clore

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax