26S proteasome non-ATPase regulatory subunit 4, Ubiquilin-2

Structure of hRpn10 Bound to UBQLN2 UBL Illustrates Basis for Complementarity between Shuttle Factors and Substrates at the Proteasome.

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0.6 mM [U-100% 15N] hRpn10 196-306, 1.38 mM UBQLN2 UBL, 20 mM NaPO4,
50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 95% H2O/5% D2O

0.6 mM [U-100% 13C] hRpn10 196-306, 1.38 mM UBQLN2 UBL, 20 mM NaPO4,
50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 100% D2O

0.6 mM [U-100% 15N; U-100% 2H] hRpn10 196-306, 1.38 mM UBQLN2 UBL, 20 mM NaPO4,
50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 95% H2O/5% D2O

0.6 mM [U-100% 13C; U-100% 15N] UBQLN2 UBL, 0.6 mM hRpn10 196-306, 20 mM NaPO4,
50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 95% H2O/5% D2O

0.6 mM [U-100% 13C; U-100% 15N] UBQLN2 UBL, 0.6 mM hRpn10 196-306, 20 mM NaPO4,
50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 100% D2O

0.6 mM [U-100% 13C; U-100% 15N] UBQLN2 UBL, 20 mM NaPO4, 50 mM sodium chloride,
2 mM DTT, 0.1 % sodium azide, 100% D2O

Schwieters, Kuszewski, Tjandra and Clore
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax