E2 conjugating enzyme UbcH7 linked via isopeptide to ubiquitin

Assignments were made by comparing to BMRB 15498, 4769 and published literature.

Synergistic recruitment of UbcH7~Ub and phosphorylated Ubl domain triggers parkin activation.

Residues 201-276 are ubiquitin.
The construct used included an SNA tag at the beginning of the ubiquitin sequence that was left over from
TEV cleavage but is not present in the chemical shift file.

HN backbone shifts for UbcH7 (C17S, C86K,C137S) covalently attached via K86 side chain to the C-terminal Gly CO in Ubiquitin.

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax