1H, 15N, 13C backbone resonance assignments of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2 in complex with 1,4,5,6-tetrahydro-NAD

oxidoreductase.
The FMN cofactor is oxidised in the PETNR:FMNox complex.
Enzyme cofactor in reduction oxidation reactions.
NADPH/NADP+ is a reducing/oxidising agent.
NADH4 is a unreactive analogue of NADH.
The related compound is tetrahydro-NAD (NADH4) in PubChem:
https://pubchem.ncbi.nlm.nih.gov/compound/169266
The difference is the 2'-phosphate group on the ribose ring of the adenosine group.

crystal form of PETNR:FMNox:NADH4 complex

TROSY offset: amide protons;
TROSY offset: amide nitrogens;
2H isotope effect: backbone CA;
2H isotope effect: backbone CB.

[(2R,3S,4S)-5-[7,8-dimethyl-2,4-bis(oxidanylidene)benzo[g]pteridin-10-yl]-2,3,4-tris(oxidanyl)pentyl] dihydrogen phosphate

Nonequivalence of Second Sphere "Noncatalytic" Residues in Pentaerythritol
Tetranitrate Reductase in Relation to Local Dynamics Linked to H-Transfer
in Reactions with NADH and NADPH Coenzymes

Recombinant PETNR is expressed and purified as a tight non-covalently bound PETNR:FMNox complex

The FMN cofactor is oxidised in the PETNR:FMNox complex

pentaerythritol tetranitrate reductase (PETNR)

pentaerythritol tetranitrate reductase (PETNR) in complex with 1,4,5,6-tetrahydro-nicotinamide-adenine-dinucleotide

1H, 15N, 13C backbone resonance assignments of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2 in complex with oxidised flavin mononucleotide

1H, 15N, 13C backbone resonance assignments of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2 in complex with 1,4,5,6-tetrahydro-NADP

WILD-TYPE PENTAERYTHRITOL TETRANITRATE REDUCTASE CONTAINING A C-TERMINAL 8-HISTIDINE TAG FROM ENTEROBACTER CLOACAE

STRUCTURE OF OXIDISED PENTAERYTHRITOL TETRANITRATE REDUCTASE FROM ENTEROBACTER CLOACAE