Backbone and side-chain methyl relaxation rates, methyl order parameters, and stereospecific resonance assignments for Zn(II) AdcR

structure of the Zn-bound repressor that is examined in this study

Methyl sidechain assignment HMCMCGCBCA following Tugarinov & Kay (J Am Chem Soc Vol 125, p.13874, 2003).

Tuning site-specific dynamics to drive allosteric activation in a pneumococcal zinc uptake regulator.

2D 1H-13C CPMG HMQC relaxation dispersion

pseudo 3D Trosy Constant Relaxation Time CPMG experiment for NH

Methyl order parameter data recorded using the latest version of the experiments
by Tugarinov, Sprangers, Kay (J.Am.Chem.Soc. 129,1743). Slow-timescale methyl dynamics
recorded using the latest version of the experiments by Korzhnev, Kay, et al.
(J.Am.Chem.Soc. 126, 3964).
Methyl sidechain assignment HMCMCGCBCA following Tugarinov & Kay (J Am Chem Soc Vol 125, p.13874, 2003)

Residues 12, 18, 19, 29, 40, 41, 51, 61, 86, 92, 134, 135, 137, and 141 should be excluded or used with caution due to resonance overlap.

Residues 12, 18, 19, 29, 40, 41, 51, 61, 86, 92, 134, 135, 137, and 141
should be excluded or used with caution due to resonance overlap.

N15_CPMG_Rex_NH_trosy_antitrosy_lek_600_v4.
Residues 12, 18, 19, 29, 40, 41, 51, 61, 86, 92, 134, 135, 137, and 141
should be excluded or used with caution due to resonance overlap.

Slow-timescale methyl dynamics recorded using the latest version of the experiments by Korzhnev, Kay, et al.
(J.Am.Chem.Soc. 126, 3964).
The following methyls should be excluded or used with caution due to resonance overlap:
CD2 of residues 12, 75, 81, 117, and 141
CD1 of residues 17, 57, 75, 97, and 117.

N15_CPMG_Rex_NH_trosy_antitrosy_lek_600_v4 was used for obtaining Rex.
The R2 values were measured directly, rather than fit along with Rex.
Residues 12, 18, 19, 29, 40, 41, 51, 61, 86, 92, 134, 135, 137, and 141
should be excluded or used with caution due to resonance overlap.

Methyl order parameter data recorded using the latest version of the experiments
by Tugarinov, Sprangers, Kay (J.Am.Chem.Soc. 129,1743).

Backbone and methyl side chain assignments of apo AdcR at pH 6.0

Backbone and methyl side chain assignments of Zn(II) AdcR at pH 6.0

Backbone and side-chain methyl relaxation rates, methyl order parameters, stereospecific resonance assignments, and relaxation rates for apo-AdcR

Crystal Structure of the zinc-dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-bound State

Crystal Structure of Adhesin competence repressor (AdcR) from Streptococcus pyogenes (C-terminally His tagged)

Crystal Structure of Adhesin competence repressor (AdcR) from Streptococcus pyogenes

Structure of Lactococcus lactis ZitR, wild type

Structure of Lactococcus lactis ZitR, C30S mutant

Structure of Lactococcus lactis ZitR, E41A mutant

Structure of Lactococcus lactis ZitR, C30AH42A mutant

Structure of Lactococcus lactis ZitR, C30AH42A mutant in apo form

Structure of Lactococcus lactis ZitR, wild type in complex with DNA

Structure of Lactococcus lactis ZitR, C30S mutant in complex with DNA

Protein expressed in 100% D2O using 2H, 12C glucose as carbon source,
15N ammonium chloride as nitrogen source, with added 1H,13C-methyl-labeled
alanine and methionine, and alphaketoisovalerate and alphaketobutyrate for
labeling ILV methyls. alphaketoisovalerate is CDLM-7317 from Cambridge
Isotope Laboratories, with 2H and 12C except at one of the two methyls,
which is 1H and 13C. alpha-ketobutyrate is CIL CDLM-7318, with 2H and 12C
except at the methyl, which is 1H and 13C.

Protein expressed in 100% D2O using 2H, 12C glucose as carbon source,
15N ammonium chloride as nitrogen source, with added alphaketoisovalerate
and alphaketobutyrate for labeling ILV methyls. alphaketoisovalerate is
CDLM-8100 from Cambridge Isotope Laboratories, with 2H everywhere except
for one methyl group, and 13C everywhere except for the other methyl group.
alpha-ketobutyrate is CIL CDLM-4611, entirely 13C, with 2H everywhere except
at the methyl, which is 1H.

10 percent 13C for stereospecific assignments

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax