Chemical shifts of amorphous-looking aggregates of the P23T mutant of human gamma-D-crystallin formed at pH 7.

Aggregated state formed at neutral pH, with possibly correlations to cataract formation.

Cataract-associated P23T γD-crystallin retains a native-like fold in amorphous-looking aggregates formed at physiological pH.

Partial chemical shift assignments of amorphous-looking aggregates
of the P23T mutant of human gamma-D-crystallin formed at pH 7.
Measurements performed by magic-angle-spinning solid-state NMR.

Solution NMR chemical shifts of cataract-causing P23T mutant of HgD crystallin in solution.

Solution NMR structure of cataract-causing P23T mutant of HgD crystallin in solution.

Crystal structure of cataract-causing P23T mutant of HgD crystallin obtained by X-ray crystallography.

Amorphous-looking aggregates (by EM) formed at pH 7

Amorphous-looking aggregates (based on TEM) formed at pH 7 in 100 mM sodium
phosphate buffer containing 5 mM dithiothreitol and 0.02% (wt/vol) sodium
azide.

The CCPN NMR assignment and data analysis application

Department of Biochemistry, Cambridge CB2 1GA, UK