side-chain methyl order parameters, stereospecific resonance assignments, and relaxation rates for apo CzrA

Proceedings of the National Academy of Sciences of the United States of America
Entropy redistribution controls allostery in a metalloregulatory protein.

2D 1H-13C CPMG HMQC relaxation dispersion

Methyl order parameter data recorded using the latest version of the experiments
by Tugarinov, Sprangers, Kay (J.Am.Chem.Soc. 129,1743). Slow-timescale dynamics
recorded using the latest version of the experiments by Korzhnev, Kay, et al.
(J.Am.Chem.Soc. 126, 3964).

Resonance assignments of CzrA in apo form

side-chain methyl order parameters, stereospecific resonance assignments, and relaxation rates for Zn(II) CzrA

Resonance assignments of CzrA in complex with Zinc ion

Resonance assignments of CzrA in complex with DNA

crystal structure of CzrA in Zn(II) form

Solution structure of CzrA in the DNA bound state

Soluction structure of CzrA in the Zn(II) state

Protein expressed in 100% D2O using 2H, 12C glucose as carbon source,
15N ammonium chloride as nitrogen source, with added 1H,13C-methyl-labeled
alanine and methionine, and alphaketoisovalerate and alphaketobutyrate for
labeling ILV methyls.

10 percent 13C for stereospecific assignments

[U-12C; U-15N; U-2H; Ala, Met, Leu, Val, Ile-d methyl 100%-1H; Ala, Met, Leu, Val, Ile-d methyl 100%-13C]

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax