1H, 13C and 15N chemical shift assignments for PARP-1 F1F2F3 domains in complex with a DNA single-strand break

All amide group chemical shifts derived from TROSY data have been corrected by adding [1J(NH)]/2 in the 1H dimension and subtracting [1J(NH)]/2 in the 15N dimension

separate tube (no insert) similar to the experimental sample tube

Structural Basis of Detection and Signaling of DNA Single-Strand Breaks by Human PARP-1.

PARP-1 F1F2F3 DNA complex. F1F2F3 was sortase ligated (N.B. additional residues LPETGG inserted between residues 214 and 215; this sample was not used for making any assignments of residues in this region, which is in the flexible linker between domains).

Labelling for residues 1-214 (and LPET of insertion):
uniform [2H,15N,13C]

Labelling for residues 215-362 (and GG of insertion):
natural abundance

PARP-1 F1F2F3 DNA complex. F1F2F3 was sortase ligated (N.B. additional
residues LPETGG inserted between residues 214 and 215; this sample was not used
for making any assignments of residues in this region, which is in the flexible
linker between domains).
Labelling for residues 1-214 (and LPET of insertion):
natural abundance
Labelling for residues 215-362 (and GG of insertion):
uniform [2H,15N,13C]

PARP-1 F1F2F3 DNA complex.
F1F2F3 15N,13C,2H labelled

Structural basis of detection and signaling of DNA single-strand breaks by human PARP 1

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax