Backbone 1H, 13C, and 15N chemical shift assignments for the double mutant G89A,D91R of KaiB in complex with the CI domain of KaiC from the Thermosynechococcus elongatus BP-1 cyanobacterial species

G89A,D91R double mutant of KaiB bound to CI

Circadian rhythms. A protein fold switch joins the circadian oscillator to clock output in cyanobacteria.

Bruker 600 MHz AVANCE III spectrometer equipped with a TCI cryoprobe and z-axis pulsed-field gradient capability

D91R single mutant of dimeric KaiB (thioredoxin-like fold)

Sample: [N15,C13,H2]-FLAG-TeKaiB-G89A-D91R-FLAG (650 uM) + FLAG-TeKaiC-17-247-R41A-K173A-FLAG (650 uM);
Buffer: 25 mM Tris, 64 mM NaCl, 0.8 mM MgCl2, 0.8 mM ADP, pH 7.0, 10 uM DSS, 0.02% NaN3, 95%H2O/5%D2O, 1X Protease Inhibitor Cocktail;
Volume: 350 uL;
Tube: shaped

robust automatic backbone assignment of proteins

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax