Backbone 1H, 13C, and 15N chemical shift assignments for the thioredoxin-like fold of the single mutant D91R of dimeric KaiB from the Thermosynechococcus elongatus BP-1 cyanobacterial species

D91R single mutant of dimeric KaiB (thioredoxin-like fold)

Circadian rhythms. A protein fold switch joins the circadian oscillator to clock output in cyanobacteria.

Bruker 600 MHz AVANCE III spectrometer equipped with a TCI cryoprobe and z-axis pulsed-field gradient capability

G89A,D91R double mutant of KaiB bound to CI

Sample: [N15,C13]-FLAG-TeKaiB-1-94-Y8A-Y94A-D91R-FLAG (1819 uM);
Buffer; 20 mM Tris, 100 mM NaCl, pH 7.0, 10 uM DSS, 0.02% NaN3, 95%H2O/5%D2O;
Volume: 320 uL;
Tube: shaped

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax