Backbone 1H, 13C, and 15N chemical shift assignments for the ground state-like fold of the single mutant G89A of dimeric KaiB from the Thermosynechococcus elongatus BP-1 cyanobacterial species

Circadian rhythms. A protein fold switch joins the circadian oscillator to clock output in cyanobacteria.

Bruker 600 MHz AVANCE III spectrometer equipped with a TCI cryoprobe and z-axis pulsed-field gradient capability

D91R single mutant of dimeric KaiB (thioredoxin-like fold)

G89A,D91R double mutant of KaiB bound to CI

Sample: [N15,C13]-FLAG-TeKaiB-1-94-Y8A-Y94A-G89A-FLAG (1760 uM);
Bufffer: 20 mM Tris, 100 mM NaCl, pH 7.0, 10 uM DSS, 0.02% NaN3, 95%H2O/5%D2O;
Volume: 320 uL;
Tube: shaped

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax