Solution structure of Blo t 19, a minor dust mite allergen from Blomia tropicalis.

Blo t 19, a minor dust mite allergen from Blomia tropicalis

Resonance for residues between 59 to 64 were degenerate. The strong intensity population is called Major Conformer.
Tolerance value used in automated NOESY assignments.

Resonance for residues between 59 to 64 were degenerate. The weak intensity population is called Minor Conformer.

separate tube (no insert) similar to the experimental sample tube

Residue 1 and 2 represent a non-native tag.

Blo t 19 allergen, partial [Blomia tropicalis]

Protein was expressed as GST-fusion and purified by affinity chromatography. It was refolded by glutathione redox couple, subject to proteolysis and finally separated from GST by gel filtration.

House dust mite Blomia tropicalis (Taxon identifier 40697)

The data from this entry were used to determine structure of Blo t 19. Due to
Proline isomerization, Blo t 19 spectrum has degenerate resonances for a stretch
of six residues in its C-terminus. These two populations have varying
intensities and are unambiguously assigned as major and minor population.
Solution structure is calculated for the Major population.

NMR data was acquired at 295K using Shigemi NMR tubes.

torsion angle dynamics, DGSA-distance geometry simulated annealing

15N Blo t 19 in Pf1 phage alignment medium.

Schwieters, Kuszewski, Tjandra and Clore