Solution Structure of NusE (S10) from Thermotoga maritima

Journal of biomolecular structure & dynamics
The two domains of Mycobacterium tuberculosis NusG protein are dynamically independent.

Replacement of ribosomal binding loop (wild type sequence region 46-67) by a single serine to improve solubility and stability

construct used for structure determination:

Ser1-Met2:Glu46-Ser47-Arg88_Leu88

Ser1 (cloning artefact from protease cleveage site)

Met2:Glu46 (corresponds to Met1:Glu45 of wild type sequence

Ser47 (replacement of ribosomal binding loop)

Arg48:Leu82 (corresponds to Arg68:Leu102 of wild type sequence)

Schwieters, Kuszewski, Tjandra and Clore