approximate digital resolution of spectra

NMR assignments for the cis and trans forms of the hemolysin II C-terminal domain.

C-terminal domain of hemolysin II from Bacillus cereus

The domain studied in this work runs from amino acids D1 to I94 (numbering scheme provided for NMR assignments) corresponding to amino acids D319 to I412 of the full-length B. Cereus hemolysin II. NMR spectra show split peaks due to cis/trans isomerization of the sole proline at position 87 for about 50% of the residues in the domain. This entry contains NMR assignments for the major cis form of the domain with the wt sequence.

NMR experiments were performed on protein samples in 20 mM sodium phosphate, pH 6.0, 1 mM EDTA, 1 mM AEBSF, and 0.05% w/v sodium azide.

The CCPN NMR assignment and data analysis application

Department of Biochemistry, Cambridge CB2 1GA, UK