Solution NMR Structure de novo designed rossmann 2x2 fold protein, Northeast Structural Genomics Consortium (NESG) Target OR16

Principles for designing ideal protein structures.

Solution Nmr Structure Of De Novo Designed Rossmann 2x2 Fold Protein, Northeast Structural Genomics Consortium Target Or16

3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY

distance geometry, simulated annealing, torsion angle dynamics, molecular dynamics

alignment medias: peg for Ori 1 and phage for Ori 2.
# Orientation Magnitude Rhombicity ORI residue number
1 -7.005 0.392 118
2 10.199 0.418 125

alignment medias: peg for Ori 1 and phage for Ori 2.
# Orientation Magnitude Rhombicity ORI residue number
# 2 -12.87 0.278 125

0.859 mM OR16.013, 90% H2O/10% D2O
alignment media peg or phage

Northeast Structural Genomics Consortium

NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG)

refinemen,structure solution,geometry optimization

refinement,geometry optimization,structure solution

data analysis,peak picking,chemical shift assignment

Zimmerman, Moseley, Kulikowski and Montelione

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

Brunger, Adams, Clore, Gros, Nilges and Read