Solution Structure of the PilZ Domain Protein PA4608 Complex with Cyclic Di-GMP Identifies Charge Clustering as Molecular Readout

single-domain PilZ protein PA4608 in complex with dimeric c-di-GMP

Information obtained from PDB's Chemical Component Dictionary
at http://wwpdb-remediation.rutgers.edu/downloads.html
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c-di-GMP, Cyclic diguanosine monophosphate

2D 1H-1H NOESY (filtered against H bound to 13C, 15N)

the two c-di-GMP molecules bind as a intercalated dimer and are in slow exchange with the free form

Solution Nmr Structure Of Pseudomonas Aeruginosa Protein Pa4608. Northeast Structural Genomics Target Pat7

Solution Structure Of The Pilz Domain Protein Pa4608 Complex With C- Di-Gmp Identifies Charge Clustering As Molecular Readout

hypothetical protein NCGM2_0983 [Pseudomonas aeruginosa NCGM2.S1]

hypothetical protein NCGM1900_2451 [Pseudomonas aeruginosa]

hypothetical protein NCGM1984_5169 [Pseudomonas aeruginosa]

hypothetical protein PA257_5789 [Pseudomonas aeruginosa]

cyclic diguanosine monophosphate-binding protein PA4608 [Pseudomonas aeruginosa]

hypothetical protein PLES_49931 [Pseudomonas aeruginosa LESB58]

hypothetical protein PA18A_562 [Pseudomonas aeruginosa 18A]

hypothetical protein P38_5109 [Pseudomonas aeruginosa MH38]

hypothetical protein PAMH27_5249 [Pseudomonas aeruginosa MH27]

type IV pilus assembly PilZ [Pseudomonas aeruginosa PA38182]

hypothetical protein PA4608 [Pseudomonas aeruginosa PAO1]

conserved hypothetical protein [Pseudomonas aeruginosa UCBPP-PA14]

hypothetical protein PSPA7_5248 [Pseudomonas aeruginosa PA7]

hypothetical protein PAM18_4702 [Pseudomonas aeruginosa M18]

hypothetical protein PADK2_24475 [Pseudomonas aeruginosa DK2]

MULTISPECIES: Cyclic diguanosine monophosphate-binding protein PA4608 [Pseudomonas]

RecName: Full=Cyclic diguanosine monophosphate-binding protein PA4608; Short=c-di-GMP-binding protein PA4608; AltName: Full=Pil

Grzesiek group, Biozentrum, University of Basel

13C-{13Cg}spin-echo difference H-15N HSQC

15N-{13Cg} spin-echo diference H-15N HAQC

0.8 mM 15N-13C labeled Protein sample in complex with unlabeled c-di-GMP

1.1mM 15N-13C labeled PA4608
without ligand

15N-13C labeled PA4608,
unlabeled c-di-GMP

0.6 mM 15N-13C-PA4608
15N-13C c-di-GMP
in PF1 phage 18mg/ml

15N PA4608
0.5 equivalent 15N-13C c-di-GMP and 0.5 equivalent unlabeled c-di-GMP

0.5mM 15N labeled PA4608
without ligand
with Pf1 phage 26mg/ml

Schwieters, Kuszewski, Tjandra and Clore
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax