Integrin beta3 subunit in a disulfide linked alphaIIb-beta3 cytosolic domain

separate tube (no insert) similar to the experimental sample tube

Proceedings of the National Academy of Sciences of the United States of America
NMR analysis of the alphaIIb beta3 cytoplasmic interaction suggests a mechanism for integrin regulation.

platelet Glycoprotein IIb (GPIIb), partial [Homo sapiens]

Solution Structure Of The Cytoplasmic Domain Of The Integrin Alpha-Iib Subunit

Platelet Integrin Alfaiib-Beta3 Cytoplasmic Domain

Nmr Structure Of The Cytoplasmic Domain Of Integrin Aiib In Dpc Micelles

The Structure Of Filamin Repeat 21 Bound To Integrin

integrin, alpha 2b [synthetic construct]

platelet glycoprotein IIb (648 AA) [Homo sapiens]

glycoprotein IIb, partial [Homo sapiens]

platelet membrane glycoprotein IIb [Homo sapiens]

glycoprotein IIb, partial [Papio cynocephalus]

Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) [Homo sapiens]

integrin alpha-IIb preproprotein [Homo sapiens]

integrin alpha-IIb precursor [Equus caballus]

PREDICTED: integrin alpha-IIb [Macaca mulatta]

PREDICTED: integrin alpha-IIb isoform X2 [Pan troglodytes]

RecName: Full=Integrin alpha-IIb; AltName: Full=GPalpha IIb; Short=GPIIb; AltName: Full=Platelet membrane glycoprotein IIb; Alt

RecName: Full=Integrin beta-3; AltName: Full=Platelet membrane glycoprotein IIIa; Short=GPIIIa; AltName: CD_antigen=CD61; Flags

TPA: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) [Bos taurus]

Platelet Integrin Alfaiib-Beta3 Transmembrane-Cytoplasmic Heterocomplex

integrin beta3 subunit [Rattus norvegicus]

plate glycoprotein IIIa (GPIIIa) [Homo sapiens]

glycoprotein IIIa precursor [Homo sapiens]

platelet glycoprotein IIIa, partial [Homo sapiens]

beta 3 integrin, GPIIIA [rats, Peptide Partial, 723 aa]

integrin beta-3 precursor [Homo sapiens]

integrin beta-3 precursor [Canis lupus familiaris]

integrin beta-3 precursor [Equus caballus]

integrin beta-3 precursor [Oryctolagus cuniculus]

The alphaIIb subunit was expressed in the pET-16b vector as a C-terminal fusion with the designed protein alpha3D, a small, highly stable peptide that expresses well in E. coli and is unrelated to integrin alphaIIb. The alphaIIb fragment was subsequently cleaved from the fusion protein using thrombin.

The beta3 subunit was expressed as a C-terminal fusion to the pET-15b histidine tag.

10% 13C used to distinguish pro-chiral methyls in valine and leucine side chains.

Schwieters, Kuszewski, Tjandra and Clore