NMR assignment of T35S/Q61L mutant of human K-Ras4b(1-169) bound to GppNHp

PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

[[[[(2R,3S,4R,5R)-5-(2-amino-6-oxo-1H-purin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]amino]phosphonic acid

NMR 1H, 13C, 15N backbone resonance assignments of the T35S and oncogenic T35S/Q61L mutants of human KRAS4b in the active, GppNHp-bound conformation.

GTPase, Cell Proliferation, Cancer, oncogenic mutant in state 1, HSQC, NMR, GppNHp

NMR assignments were carried out using oncogenic K-Ras4b construct that harbors T35S, Q61L, and C118S mutants.

T35S mutant of K-Ras4b(1-169) bound to GppNHp

20mM MES, 50mM NaCl, 100mM KCl, 2mM MgCl2, 1mM TCEP, pH 6.5, 0.02% NaN3