Solution NMR structure of fold-Z Gogy; de novo designed protein with an asymmetric all-alpha topology

Chemical shift reference of 1H was based on the proton of water (4.784ppm at 303K) and then those of 15N and 13C were calculated based on their gyro-magnetic ratios.

Design of complicated all-α protein structures.

Solution NMR structure of fold-0 Chantal; de novo designed protein with an asymmetric all-alpha topology

Solution NMR structure of fold-C Rei; de novo designed protein with an asymmetric all-alpha topology

Solution NMR structure of fold-K Mussoc; de novo designed protein with an asymmetric all-alpha topology

Solution NMR structure of fold-U Nomur; de novo designed protein with an asymmetric all-alpha topology

0.9 mM [U-100% 13C; U-100% 15N] Gogy, 6.8 mM potassium phosphate, 3.20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

0.2 mM [U-100% 13C; U-100% 15N] Gogy, 6.8 mM potassium phosphate, 3.2 mM sodium phosphate, 50 mM sodium chloride, 7 mg/mL Pf1 phage, 95% H2O/5% D2O

Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax