Solution NMR structure of human RAD18 (198-240) in complex with ubiquitin

Polyubiquitin-B, E3 ubiquitin-protein ligase RAD18 (E.C.2.3.2.27)

Mechanisms of Ubiquitin-Nucleosome Recognition and Regulation of 53BP1 Chromatin Recruitment by RNF168/169 and RAD18.

RING-type E3 ubiquitin transferase RAD18

0.6 mM [U-100% 13C; U-100% 15N] RAD18, 3 mM Ubiquitin, 25 mM MES-Bis-TRIS,
50 mM KCl, 10 uM ZnCl2, 90% H2O/10% D2O.

3 mM RAD18, 0.6 mM [U-100% 13C; U-100% 15N] Ubiquitin, 25 mM MES-Bis-TRIS,
50 mM KCl, 10 uM ZnCl2, 90% H2O/10% D2O.

0.9 mM [U-15N] RAD18, 25 mM MES-Bis-TRIS, 50 mM KCl,
10 uM ZnCl2, 90% H2O/10% D2O.

0.2 mM [U-100% 15N] RAD18, 1.0 mM Ubiquitin, 25 mM MES-Bis-TRIS,
50 mM KCl, 10 uM ZnCl2, 5 % Alkyl-polyethylene glycol
(C12E5)/n-hexanol mixture, 90% H2O/10% D2O.

1.0 mM RAD18, 0.2 mM [U-100% 15N] Ubiquitin, 25 mM MES-Bis-TRIS,
50 mM KCl, 10 uM ZnCl2, 5 % Alkyl-polyethylene glycol
(C12E5)/n-hexanol mixture, 90% H2O/10% D2O.

Alkyl-polyethylene glycol (C12E5)/n-hexanol mixture

Schwieters, Kuszewski, Tjandra and Clore