Solution NMR Structure of Heat shock factor protein 1 DNA binding domain from homo sapiens, Northeast Structural Genomics Consortium Target HR3023C

3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY

Northeast Structural Genomics Consortium Target HR3023C

Solution Nmr Structure Of Heat Shock Factor Protein 1 Dna Binding Domain From Homo Sapiens, Northeast Structural Genomics Conso

heat shock transcription factor 1 variant [Homo sapiens]

heat shock transcription factor 1 [synthetic construct]

heat shock factor protein 1 [Pan troglodytes]

heat shock transcription factor 1 [Mus musculus]

heat shock transcription factor 1 [Bos taurus]

Heat shock transcription factor 1 [Homo sapiens]

heat shock factor protein 1 [Bos taurus]

heat shock factor protein 1 [Sus scrofa]

heat shock transcription factor 1 [Capra hircus]

heat shock factor protein 1 [Homo sapiens]

RecName: Full=Heat shock factor protein 1; Short=HSF 1; AltName: Full=Heat shock transcription factor 1; Short=HSTF 1

TPA: heat shock factor protein 1 [Bos taurus]

distance geometry, molecular dynamics, simulated annealing, torsion angle dynamics

NMR buffer 6.5 -- 0.02% NaN3, 10mM DTT, 5mM CaCL2, 100mM NaCL, 1x Proteinase Inhibitors, 20mM MES pH 6.5, 10% D2O, 50uM DSS

NMR buffer 6.5 -- 0.02% NaN3, 10mM DTT, 5mM CaCL2, 100mM NaCL, 1x Proteinase Inhibitors,
20mM MES pH 6.5, 10% D2O, 50uM DSS
aligned in Positively Charged Compressed Poly Acrylamide Gel.

NMR buffer 6.5 -- 0.02% NaN3, 10mM DTT, 5mM CaCL2, 100mM NaCL, 1x Proteinase Inhibitors,
20mM MES pH 6.5, 10% D2O, 50uM DSS
aligned in Peg/hexanol.

Northeast Structural Genomics Consortium

NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG)

refinemen,structure solution,geometry optimization
refinement,geometry optimization,structure solution
data analysis,peak picking,chemical shift assignment
Zimmerman, Moseley, Kulikowski and Montelione
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Brunger, Adams, Clore, Gros, Nilges and Read